【关键词】 蛛网膜下腔出血；脑损伤；舒芬太尼；炎症反应；氧化应激；细胞凋亡；大鼠
【文章编号】 1009-153X（2021）07-0531-06 【文献标志码】 A 【中国图书资料分类号】 R 743.9
Effect of sufentanil on brain injury after subarachnoid hemorrhage in adult rats ZHOU You-dong, MA Jin-yang, HUANG Song, HU Huo-jun, FU Chang-tao, YUAN Gao, GUO Jin-man, WANG Lei. Institute of Neurology, China Three Gorges University; Department of Neurosurgery, Yichang Central People's Hospital, Yichang 441021, China 【Abstract】 Objective To explore the effect of sufentanil on the brain injury after subarachnoid hemorrhage (SAH) in adult rats. Methods Sixty adult SD rats were randomly divided into sham operation group, model group and sufentanil group, with 20 rats in each group. The SAH model was established by intravascular perforation. The rats of sufentanil group were injected with sufentanil for 21 days (5 μg/kg, once a day) 6 hours after SAH, and the rats of sham operation and model groups were injected with the same amount of normal saline. The modified modified neurological severity scale (mNSS) score was used to assess neurological function 1, 2 and 21 days after SAH. After the final neurofunction evaluation, the wet-to-dry weight ratio method was used to assess the brain water content, TUNEL staining was used to detect the neuronal apoptosis rate, EILSA method was used to determine the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH- PX), interleukin (IL) - 1β, IL- 6 and tumor necrosis factor (TNF- α), and western blotting was used to detect the protein expresson of nuclear factor E2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), nuclear transcription factor-κB (NF-κB) p65, B-cell lymphoma 2 (BCL-2) and cleaved-caspase-3. Results Compared with the sham operation group, the mNSS score, the brain water content, the neuronal apoptosis rate, and the levels of MDA, TNF-α, IL-1β, IL-6, NF-kB p65 and cleaved-caspase-3 were significantly increased in the model group (P<0.05), while the levels of SOD, GSH-Px, BCL-2, Nrf2 and HO-1 were significantly reduced (P<0.05). Compared with the model group, the above-mentioned changes in the sufentanil group were significantly improved (P<0.05). Conclusions Shufentanyl has a protective effect on the brain injury after SAH in adult rats, which may be related to the anti-apoptosis, antioxidant stress and anti-inflammatory effects of shufentanyl. 【Key words】 Subarachnoid hemorrhage; Brain injury; Sufentanil; Inflammation; Oxidative stress; Cell apoptosis; Rat
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舒芬太尼对大鼠蛛网膜下腔出血后脑损伤的保护作用_周有东.pdf |
