【关键词】 脑缺血再灌注损伤；七叶皂苷钠；炎症反应；氧化应激反应；细胞凋亡
【文章编号】 1009-153X（2021）08-0616-06 【文献标志码】 A 【中国图书资料分类号】 R 743
Protective effect of sodium aescinate on adult rats after cerebral ischemia-reperfusion injury WANG Lei, HU Huo- jun, MA Jin- yang, HUANG Song, DONG Yuan- xun, WANG Xu- guang, ZHOU You- dong. Institute of Neurology, China Three Gorges University; Department of Neurosurgery, Yichang Central People's Hospital, Yichang 441021, China 【Abstract】 Objective To explore the effect of sodium aescinate on the adult rats after cerebral ischemia- reperfusion injury (CIRI). Methods Sixty adult SD rats were randomly divided into sham operation group, model group and aescinate group, with 20 rats in each group. A rat model of focal middle cerebral artery occlusion and reperfusion injury was established by the modified Zea- Longa method. The aescinate group was intraperitoneally injected with sodium aescinate (2.8 mg/kg) 1 hour after the injury, and the sham operation and model groups were intraperitoneally injected with saline. Twenty-four hours after the injury, the Longa score was used to evaluate the neurological damage, the weighing method was used to detect the degree of brain edema, the ELISA method was used to detect the levels of superoxide dismutase (SOD) and catalase (CAT), malondialdehyde (MDA), tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β in the injured brain tissues, the TUNEL method was used to detect the neuronal apoptosis rate in the injured brain tissues, and the Western blotting was used to detect the expression levels of dual-specific phosphatase 1 (DUSP1), nuclear transcription factor-κB (NF-κB) p65, Bcl-2 and Bax in the damaged brain tissues. Results after CIRI, the Longa score was significantly increased (P<0.01), the brain water content, the neuronal apoptosis rate, the levels of MDA, TNF- α, IL-1β, IL-6 NF- kB p65 and Bax were significantly increased in the injured cerebral tissues (P<0.001), and the levels of SOD, CAT, DUSP1 and Bcl- 2 were significantly reduced in the injured cerebral tissues (P<0.001). Sodium aescinate significantly reversed the above reactions in the CIRI rats (P<0.05). Conclusions Sodium aescinate significantly improves the CIRI in adult rats, and the mechanism may be related to reducing inflammation, oxidative stress, and neuronal apoptosis. 【Key words】 Cerebral ischemia-reperfusion injury; Aescinate; Adult rat; Inflammation; Oxidative stress; Neuronal apoptosis
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七叶皂苷钠对大鼠脑缺血再灌注损伤的保护作用_汪雷.pdf |
